P-172: Teratogenic Effects of Imidacloprid and Precocene1 on Reproductive Ability in Female Wistar Rats

Background: Embryonic development is one of the most critical stages in animal life. This step is strongly influenced by environmental and internal factors. The widespread uses of agricultural pesticide and their teratogenic effect on fetuses is one of the biggest health challenges facing the human. We examined the effects of Imidacloprid (IMI) and Precocene1 (PRE1) on reproductive ability in Wistar rats. Materials and Methods: 15 female adult rats were maintained under standard conditions and their gestation were confirmed by observation of the vaginal plagues (VP). From seventh up to twentieth days of gestation, IMI and PRE1 were injected intravenously at 10mg/kg concentration. Fourteen days after birth their blood were collected through the heart of newborn rats. The analysis of Estradiol, Testosterone, Progesterone, LH and FSH in blood samples was done by electrochemical luminescence technique. Number of infants after they reach the age of fertility tested for reproductive ability. Waiting times for intercourse and the number of litter born was considered. Results: Estradiol level in female rats which treated with IMI, PRE1 and IMI + PRE1 compared to control group respectively showed 1.2, 1.25 and 1.28 percent reduction. Progesterone levels in female rats which were treated with IMI, PRE1 and IMI + PRE1 compared to control group respectively showed 1.1, 1.15 and 1.17 percent reduction. In all groups LH level was less than 0.1 mlu/ml and FSH level was less than 0.1 iu/l. Conclusion: We can consider that PRE1 which can cause sterility in some insects and IMI as an inhibitor of acetylcholine receptors which may be effective on the reproductive system through the CNS. Serological results confirm that IMI and PRE1 could have effects on infertility in Wistar rats.